Articles

Original Research

Published 4 December 2025

Development of efficient targeted insertion mediated by CRISPR-Cas12a and homology-directed repair in maize

Brenden Barco · Shujie Dong · Yuki Matsuba · Ashley Crook · Ruiji Xu · Yingxiao Zhang · Chengjin Zhang · Ryan Carlin · Kevin Potter · Stephen B. Rigoulot · Jeongmoo Park · Erin M. Seaberry · Allison Parrish · Sivamani Elumalai · Sam Nalapalli · Craig Schuller · Anna Prairie · Anna Mangum · Kangfeng Mei · Hao Wu · Melissa Murray · Kristin Setliff · Francine Johnson · Dawn McNamara · Ling Zhu · Mark Rose · Weining Gu · Hao Hu · Yuanji Zhang · Yaping Jiang · Wenling Wang · Guozhu Tang · Lizhao Geng · Jianping Xu · Wan Shi · Jason Nichols · Tim Kelliher · Liang Shi · Ian Jepson · Qiudeng Que

Frontiers in Genome Editing

doi 10.3389/fgeed.2025.1713347

Development of efficient targeted insertion mediated by CRISPR-Cas12a and homology-directed repair in maize

CRISPR-Cas9 mediated editing of starch branching enzyme, SBE2 gene in potato for enhanced resistant starch for health benefits