Decoding Fungal Physiology and Metabolism: Best Practices and Innovations

Reproducibility in fungal research depends on the consistent application of validated experimental methods — yet significant variation persists across laboratories in culture conditions, enzymatic assays, and metabolic profiling workflows. This Research Topic addresses that gap directly.We invite submissions that establish or validate standardised protocols across core areas of fungal physiology and metabolism, including: optimised and reproducible fungal culture media formulations; robust assays for enzymatic activity measurement; standardised approaches to cellular respiration and bioenergetics; and harmonised lipidomic and metabolomic workflows suited to diverse fungal species and growth conditions.Contributions may take the form of original research articles, methods articles, or registered reports. Submissions should clearly describe how protocols minimise batch-to-batch variation, define acceptance criteria for experimental reproducibility, and provide sufficient methodological detail for independent replication. We particularly welcome comparative studies across strains, species, or laboratory settings that demonstrate protocol robustness.This topic is relevant to researchers working across fundamental fungal biology, applied mycology, biotechnology, and fungal pathogenesis — wherever rigorous, transferable methods are needed to underpin experimental work. Topic editors: Francisco José Fernández Perrino Source: https://www.frontiersin.org/research-topics/83033/decoding-fungal-physiology-and-metabolism-best-practices-and-innovations