Extracellular Vesicles in Regenerative Medicine: From Mechanistic Insights to Clinical Translation

Extracellular vesicles (EVs), particularly exosomes, have emerged as critical mediators of intercellular communication and hold immense promise as next-generation therapeutics in regenerative medicine. Derived from diverse cell types, EVs encapsulate bioactive cargo—including proteins, lipids, and RNAs—that can modulate immune responses, stimulate tissue repair, and promote cellular homeostasis. Unlike traditional cell-based therapies, EVs provide a cell-free alternative with potential advantages in safety, storage, and scalability. Recent advances in EV isolation, characterization, and bioengineering have significantly expanded their translational potential. However, a comprehensive understanding of the molecular and cellular mechanisms that govern EV biology remains incomplete, limiting progress toward clinical translation.This Research Topic aims to explore the mechanistic aspects of EV biogenesis, cargo selection, and trafficking routes, emphasizing how endosomal sorting complexes, Golgi-derived vesicles, and plasma membrane dynamics regulate EV formation and release. To advance the field, it is critical to integrate insights from fundamental biology with applied technologies such as scalable production, bioengineering, targeted delivery, and biomaterial integration.We also invite exploration of intracellular membrane trafficking pathways, including the roles of the ESCRT machinery, Rab GTPases, tethering factors, and lipid remodeling. Contributions that push the frontier of EV-based therapies—from novel discoveries on how EVs influence tissue microenvironments to the development of smart delivery systems and regulatory strategies for clinical translation—are highly encouraged. By fostering interdisciplinary collaboration, this collection aims to help shape the next phase of regenerative medicine: safe, scalable, and precise EV therapeutics.This Research Topic seeks to integrate mechanistic studies of extracellular vesicle biogenesis and trafficking with advances in regenerative medicine, highlighting how fundamental organelle dynamics shape therapeutic potential. We welcome submissions in the following themes, but not limited to• Mechanistic studies elucidating EV biogenesis, cargo selection, trafficking, and regeneration• Innovations in EV isolation, characterization, and cargo profiling• Engineering and biofunctionalization of EVs for targeted tissue repair• Preclinical models evaluating EVs for wound healing, cartilage, cardiac, neural, or organ regeneration• EV integration into biomaterials (e.g., hydrogels, scaffolds)• GMP manufacturing, stability, and storage solutions for therapeutic EVs. Topic editors: Chee-Yin Wong, Badrul Hisham Yahaya, Sik Loo Tan, Patricia Severino Source: https://www.frontiersin.org/research-topics/73668/extracellular-vesicles-in-regenerative-medicine-from-mechanistic-insights-to-clinical-translation