Nano-microtechnology Enabled Immuno-Engineering and Multiscale Fabrication for Next-Generation Regenerative Medicine

Nanomedicine, biomaterials, immunoengineering, and regenerative medicine increasingly recognize that immune responses are central to the success of engineered tissues and therapeutic materials. However, many scaffolds and nanomaterials are still evaluated using static, reductionist assays that focus on isolated cell populations or limited inflammatory markers. These approaches do not adequately capture the dynamic interactions among macrophages, lymphocytes, stromal cells, vascular networks, extracellular matrix, and material interfaces that determine tissue repair. Recent advances in nano- and microfabrication, controlled delivery, three-dimensional culture, bioreactor technology, and multimodal imaging are enabling more precise regulation and observation of immune–tissue interactions. Nevertheless, important gaps remain in connecting material properties and immune mechanisms to functional regeneration in physiologically relevant models. A more integrated framework is needed to establish how engineered microenvironments shape immune behaviour over time and how these effects translate across tissue systems and toward clinical application.This Research Topic aims to advance strategies that deliberately engineer the immune microenvironment to promote tissue regeneration and to establish experimental models that reflect the spatial, temporal, and multicellular complexity of living tissues. Contributions may address how nano- and microstructured materials regulate innate and adaptive immune responses, how therapeutic payloads can be delivered with precise spatial and temporal control, and how dynamic culture systems can improve the predictive value of preclinical testing. The Research Topic also seeks to clarify which model features, imaging methods, safety assessments, and validation standards are most relevant for translating immunomodulatory regenerative platforms into clinical practice.To gather further insights into immune-focused material design and physiologically relevant models for regenerative medicine, we welcome articles addressing, but not limited to, the following themes:Nanomaterials and nano-microstructured scaffolds for regulating macrophage, T-cell, and innate immune responsesImmunomodulatory delivery systems for growth factors, drugs, gene therapies, and other therapeutic agentsNano-microengineered three-dimensional scaffolds that reproduce complex tissue architectureBioreactor systems for dynamic, spatiotemporally controlled culture and tissue maturationMultimodal imaging, including nanoparticle-based contrast agents, for monitoring cell fate, immune dynamics, and matrix remodellingInteractions among immune cells, biomaterials, extracellular matrix, vasculature, and regenerating tissuesTranslational studies, biosafety evaluation, and regulatory considerations for nanotechnology-based regenerative platformsApplications in musculoskeletal, neural, cardiovascular, and other tissue systemsPhysiologically relevant alternatives to static and simplified validation approachesStandards for evaluating immune modulation, tissue function, durability, and clinical readinessAppendix: We welcome Original Research, Brief Research Reports, Reviews, and Perspectives. Topic editors: Carlotta Pucci, Chiara Martinelli, Ajay Vikram Singh Source: https://www.frontiersin.org/research-topics/76711/nano-microtechnology-enabled-immuno-engineering-and-multiscale-fabrication-for-next-generation-regenerative-medicine