Phase Separation and Nuclear Bodies in Genome Organization​

The eukaryotic nucleus is a highly organized yet dynamic environment, where genome function depends on precise spatial and temporal control of nuclear architecture. Recent work has revealed that liquid–liquid phase separation (LLPS) and biomolecular condensation contribute substantially to this organization, driving the assembly of membraneless compartments that concentrate specific factors and reactions. Yet how these dynamic compartments form, mature, and influence genome function remains incompletely understood, particularly regarding their material properties, regulatory control, and functional consequences for chromatin and transcription.This Research Topic invites contributions that dissect the biophysical principles, molecular components, and regulatory roles of nuclear condensates and phase-separated nuclear bodies (e.g., nucleoli, speckles, Polycomb bodies, transcriptional condensates) in organizing chromatin and controlling gene expression.We particularly welcome:• Experimental and computational studies defining how LLPS and biomolecular condensation shape 3D genome architecture across multiple scales.• Mechanistic work linking condensate formation and dynamics to transcription, RNA processing, DNA repair, and replication.• Quantitative imaging, single-molecule, live-cell, computational, and AI-assisted approaches to characterize the dynamics, morphology, phase behavior, and material properties of nuclear condensates, including material-state transitions.• Perturbation studies (genetic, chemical, or optogenetic) that causally connect condensate formation, dynamics, or material-state transitions to cell fate decisions, stress responses, or disease states. Topic editors: Salla Mattola, Liang Wang Source: https://www.frontiersin.org/research-topics/85782/phase-separation-and-nuclear-bodies-in-genome-organization