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Frontiers in Neuroenergetics ·

Volume 2 - 2010 | https://doi.org/10.3389/fnene.2010.00027

General Requirement for Harvesting Antennae at Ca 2+ and H + Channels and Transporters

  • Cristián Martínez1,2
  • Dante Kalise3
  • L. Felipe Barros1,2*corresponding author
  1. 11 Centro de Estudios Científicos (CECS) Valdivia, Chile
  2. 22 Centro de Ingeniería de la Innovación del CECS (CIN) Valdivia, Chile
  3. 33 Department of Mathematics, University of Bergen Bergen, Norway
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Abstract

The production and dissipation of energy in cells is intimately linked to the movement of small molecules in and out of enzymes, channels, and transporters. An analytical model of diffusion was described previously, which was used to estimate local effects of these proteins acting as molecular sources. The present article describes a simple but more general model, which can be used to estimate the local impact of proteins acting as molecular sinks. The results show that the enzymes, transporters, and channels, whose substrates are present at relatively high concentrations like ATP, Na + , glucose, lactate, and pyruvate, do not operate fast enough to deplete their vicinity to a meaningful extent, supporting the notion that for these molecules the cytosol is a well-mixed compartment. One specific consequence of this analysis is that the well-documented cross-talk existing between the Na + /K + ATPase and the glycolytic machinery should not be explained by putative changes in local ATP concentration. In contrast, Ca2 + and H + transporters like the Na + /Ca2 + exchanger NCX and the Na + /H + exchanger NHE, show experimental rates of transport that are two to three orders of magnitude faster than the rates at which the aqueous phase may possibly feed their binding sites. This paradoxical result implies that Ca2 + and H + transporters do not extract their substrates directly from the bulk cytosol, but from an intermediate “harvesting” compartment located between the aqueous phase and the transport site.

Keywords

References

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Citation

Martínez, C., et al. (2010). General Requirement for Harvesting Antennae at Ca 2+ and H + Channels and Transporters. Frontiers in Neuroenergetics, Volume 2. https://doi.org/
Received
Accepted
Published
Volume
2 - 2010

Copyright

Copyright © 2010 Martínez, Kalise and Barros.

License: http://www.frontiersin.org/licenseagreement

*Correspondence: L. Felipe Barros

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