Correction: Automated ROI detection allows rapid quantification of synaptic activity across tens of thousands of synapses in cell culture

There was a mistake in Figure 6 as published. The x-axes for the raster plots in Figure 6C are misaligned. The corrected Figure 6 appears below.

There was a mistake in Figure 8 as published. The x-axes in Figure 8C were misaligned. The corrected Figure 8 appears below.

A correction has been made to the Figure 8 caption as published. The incorrect sentence for Figure 8A reads as, “(A) Images of maximum fluorescence minus minimum fluorescence projections of 3 min synaptic calcium imaging recordings of regions treated overnight with 15 μg/μL of either control antibody (MGO-53, left), or patient-derived NMDAR autoantibodies (003-102, 007-124, and 008-218; right; scale bar = 25 μm).” The correct sentence should read as, “(A) Images of maximum fluorescence minus minimum fluorescence projections of 3 min synaptic calcium imaging recordings of regions treated overnight with 15 μg/ml of either control antibody (MGO-53, left), or patient-derived NMDAR autoantibodies (003-102, 007-124, and 008-218; right; scale bar = 25 μm).”

A correction has been made to the Methods, Cell Culture Preparation, paragraph 1. The incorrect sentence reads as, “A total of 12 litters of rat pups were used (3 for acute treatments with GLYCINE, NBQX, ketamine, memantine, and DMSO vehicle, 4 for anti-NMDAR patient autoantibodies, and 5 for ketamine, memantine, and DMSO vehicle overnight treatments).” The correct sentence should read as, “A total of 12 litters of rat pups were used (3 for acute treatments with glycine, NBQX, ketamine, memantine, and DMSO vehicle, 4 for anti-NMDAR patient autoantibodies, and 5 for ketamine, memantine, and DMSO vehicle overnight treatments).”

A correction has been made to the Methods, Signal Extraction and Normalization, paragraph 1. The incorrect sentence reads as, “To correct for bleaching and small fluorescence fluctuations, baseline correction was performed using an adaptive iteratively reweighted penalized least squares regression (Zhang et al., 2010).” The correct sentence should read as, “To correct for bleaching and small fluorescence fluctuations, baseline correction was performed using an adaptive iteratively reweighted penalized least squares regression (airPLS; Zhang et al., 2010).”

A correction has been made to the Results, paragraph 2. The incorrect sentence reads as, “The raw fluorescence extracted from Suite2p for each ROI (Figure 1F) was then baseline corrected using an automated iterative least squares regression (adaptive iteratively reweighted penalized least squares regression; Zhang et al., 2010) and normalized (ΔF/F0) (Figure 1G).” The correct sentence should read as, “The raw fluorescence extracted from Suite2p for each ROI (Figure 1F) was then baseline corrected using an adaptive iteratively reweighted penalized least squares regression (airPLS; Zhang et al., 2010) and normalized (ΔF/F₀) (Figure 1G).”

A correction has been made to the Results, AMPA receptors have limited contribution to synaptic calcium transients, paragraph 2. The incorrect sentence reads as, “This increase likely occurs because more glutamate is available to bind NMDARs in the presence of NBQX.” This sentence should be deleted.

The original version of this article has been updated.

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