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Frontiers in Parasitology ·

Volume 4 - 2025 | https://doi.org/10.3389/fpara.2025.1711595

Correction: In vitro co-culture model of Trichomonas vaginalis, Candida albicans, and Lactobacillus crispatus: a system for assessing antimicrobial activity and microorganism interactions in vaginitis

  • Fernanda Gomes Cardoso1
  • Luisa Trindade dos Santos1
  • Saulo Almeida Menezes1
  • Graziela Vargas Rigo1
  • Tiana Tasca1*corresponding author
  1. Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
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Abstract

Keywords

In the published article, there was a mistake in **Figure 3 and Figure 5 . Figure 3 appeared in place of Figure 5** and vice versa. The corrected figures appear below.

Hemolysis of erythrocytes co-incubated with monocultures or co-cultures of *Trichomonas* vaginalis, *Candida albicans* and *Lactobacillus crispatus.* **(A)** ATCC30236 *T. vaginalis* standard isolate, *C*. *albicans* (CA3), and *L. crispatus* (LC2). **(B)** TV-LACM6 *T. vaginalis* fresh clinical isolate, *C*. *albicans* (CA3), and *L. crispatus* (LC1). Positive control of hemolysis is erythrocytes treated with 0.2% Triton X-100. Results are expressed as a percentage of total hemolysis, presented as the mean ± S.D. of at least two blood samples. The percentage of hemolysis from erythrocytes co-incubated with *T. vaginalis* monocultures was compared to co-cultures with the protozoan. (*) indicates a significant difference.
Figure 1. Hemolysis of erythrocytes co-incubated with monocultures or co-cultures of Trichomonas vaginalis, Candida albicans and Lactobacillus crispatus. (A) ATCC30236 T. vaginalis standard isolate, C. albicans (CA3), and L. crispatus (LC2). (B) TV-LACM6 T. vaginalis fresh clinical isolate, C. albicans (CA3), and L. crispatus (LC1). Positive control of hemolysis is erythrocytes treated with 0.2% Triton X-100. Results are expressed as a percentage of total hemolysis, presented as the mean ± S.D. of at least two blood samples. The percentage of hemolysis from erythrocytes co-incubated with T. vaginalis monocultures was compared to co-cultures with the protozoan. (*) indicates a significant difference.
Yeast-to-hyphal form transition of *Candida albicans* in monoculture and co-culture. **(A)** Co-culture of *C*. *albicans* (CA3, initial density at 3.33x10<sup>4</sup> CFU/mL) with ATCC *Trichomonas vaginalis* isolate (ATCC3026, initial density at 1 x 10<sup>6</sup> trophozoites/mL) and second density of *Lactobacillus crispatus* (LC2, *i*nitial density at 5.53x10<sup>6</sup> CFU/mL). **(B)** Co-culture of CA3 with fresh clinical *T. vaginalis* isolate (TV-LACM6, initial density at 1 x 10<sup>6</sup> trophozoites/mL) and first density of *L. crispatus* (LC1, initial density at 5.53x10<sup>7</sup> CFU/mL. The date were expressed by percentage de hyphae formation. Results are representative of two independent experiments conducted with triplicate assays. (*) Statistically significant difference (p < 0.05).
Figure 2. Yeast-to-hyphal form transition of Candida albicans in monoculture and co-culture. (A) Co-culture of C. albicans (CA3, initial density at 3.33x104 CFU/mL) with ATCC Trichomonas vaginalis isolate (ATCC3026, initial density at 1 x 106 trophozoites/mL) and second density of Lactobacillus crispatus (LC2, initial density at 5.53x106 CFU/mL). (B) Co-culture of CA3 with fresh clinical T. vaginalis isolate (TV-LACM6, initial density at 1 x 106 trophozoites/mL) and first density of L. crispatus (LC1, initial density at 5.53x107 CFU/mL. The date were expressed by percentage de hyphae formation. Results are representative of two independent experiments conducted with triplicate assays. (*) Statistically significant difference (p < 0.05).

The original article has been updated.

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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Citation

Cardoso, F. G., et al. (2025). Correction: In vitro co-culture model of Trichomonas vaginalis, Candida albicans, and Lactobacillus crispatus: a system for assessing antimicrobial activity and microorganism interactions in vaginitis. Frontiers in Parasitology, Volume 4. https://doi.org/
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4 - 2025

Copyright

Copyright © 2025 Cardoso, Santos, Menezes, Rigo and Tasca.

License: http://creativecommons.org/licenses/by/4.0/

*Correspondence: Tiana Tasca

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